Anti Fibrinogen (human)
mouse monoclonal antibody
Cat.No. HYB 051-07
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Fibrinogen, the precursor of fibrin, is the coagulable protein in the blood plasma of vertebrates. Fibrinogen consists as a dimer of 3 pairs of non-identical chains Aa (Mr= 66,800), Bb (Mr= 52,000) and g (Mr=46,500) cross-linked by disulfide bonds in their N-terminal segments. The molecule has 2 terminal D domains and one central E domain, all three domains are separated when fibrinogen is degradated by plasmin. MW is 340 kDa (1). Concentration in human plasma is 2-3 mg/ml (2).
Fibrinogen isolated from human plasma
HYB 051-07 has specificity for human fibrinogen degradation product D-fragment. HYB 051-07 reacts with both purified D-monomer, fibrinogen and factor VIII.
Epitope specificity differs from that of HYB 051-04 as determined by inhibition ELISA.
HYB 051-07 reacts strongly with D-monomer (Sigma, F-9036). Strong reaction is seen in ELISA with antigen directly coated onto the microtiter plate or in sandwich ELISA in combination with a polyclonal antibody against fibrinogen (DAKO A0080). In Western blotting after SDS-PAGE, HYB 051-07 reacts with fibrinogen degradation product D-monomer in nonreduced forms to create a band of approximately 92kDa.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:16000 | |
| Immunoblotting | Yes | 1:2000 | |
| Immunohistochemistry | Not determined |
1. Doolittle RF (1977) Structure and function of fibrinogen. Horiz Biochem Biophys 3:164-191.
2. Scott T & Eagleson M (1988) Concise Encyclopedia Biochemistry: Walter de Gruyter, New York.
